Journal: RSC Advances

Article Title: Heparinization of anodized aluminum oxide (AAO) membranes for affinity separations of biocolloids

doi: 10.1039/d6ra07687d

Figure Lengend Snippet: Fluorescence emission spectra of FGF2 Ab-FITC released from bioaffinity experiments. (a) Is the spectra of protein and antibody captured by AAO, NH 2 –AAO, and HEP–AAO and released in 2 M NaCl in 50 mM Tris–HCl. (b) Is the spectra of the protein and antibody captured on functionalized amine-capped membranes, RCOO–NH 2 –AAO and RCOO–HEP–AAO, and released in 2 M NaCl in 50 mM Tris–HCl, with the pH adjusted to pH 8 to prevent the reversal of the amine capping.

Article Snippet: The bioactivity of immobilized heparin was demonstrated using human FGF2 basic protein (FGF2, premium grade, Acro Biosystems) and FGF2 polyclonal antibody, FITC conjugated (Ab-FITC, Bioss Antibodies) in aqueous solutions of Tris–HCl (Biotechnology VWR) and sodium chloride (Fisher Chemical).

Techniques: Fluorescence

Journal: RSC Advances

Article Title: Heparinization of anodized aluminum oxide (AAO) membranes for affinity separations of biocolloids

doi: 10.1039/d6ra07687d

Figure Lengend Snippet: Fluorescence emission spectra of FGF2 Ab-FITC released from bioaffinity experiments. (a) Is the spectra of protein and antibody captured by AAO, NH 2 –AAO, and HEP–AAO and released in 2 M NaCl in 50 mM Tris–HCl. (b) Is the spectra of the protein and antibody captured on functionalized amine-capped membranes, RCOO–NH 2 –AAO and RCOO–HEP–AAO, and released in 2 M NaCl in 50 mM Tris–HCl, with the pH adjusted to pH 8 to prevent the reversal of the amine capping.

Article Snippet: The bioactivity of immobilized heparin was demonstrated using human FGF2 basic protein (FGF2, premium grade, Acro Biosystems) and FGF2 polyclonal antibody, FITC conjugated (Ab-FITC, Bioss Antibodies) in aqueous solutions of Tris–HCl (Biotechnology VWR) and sodium chloride (Fisher Chemical).

Techniques: Fluorescence

Journal: RSC Advances

Article Title: Heparinization of anodized aluminum oxide (AAO) membranes for affinity separations of biocolloids

doi: 10.1039/d6ra07687d

Figure Lengend Snippet: Zeta potential measurements of 20 nm SNPs. Measurements of 20 nm SNPs, SNPAs, and FITC–SNPs were conducted in triplicate over a range of pHs to analyze the change in surface charge.

Article Snippet: The bioactivity of immobilized heparin was demonstrated using human FGF2 basic protein (FGF2, premium grade, Acro Biosystems) and FGF2 polyclonal antibody, FITC conjugated (Ab-FITC, Bioss Antibodies) in aqueous solutions of Tris–HCl (Biotechnology VWR) and sodium chloride (Fisher Chemical).

Techniques: Zeta Potential Analyzer

Journal: RSC Advances

Article Title: Heparinization of anodized aluminum oxide (AAO) membranes for affinity separations of biocolloids

doi: 10.1039/d6ra07687d

Figure Lengend Snippet: Recovery of 20 nm FITC–SNP from three cycles static adsorption/desorption on HEP–AAO and NH 2 –AAO membranes to demonstrate reusability of the membranes. Percent bound based on FITC-SNPs initially available to the membrane. The error bars represent standard deviations ( n = 3 independent membranes).

Article Snippet: The bioactivity of immobilized heparin was demonstrated using human FGF2 basic protein (FGF2, premium grade, Acro Biosystems) and FGF2 polyclonal antibody, FITC conjugated (Ab-FITC, Bioss Antibodies) in aqueous solutions of Tris–HCl (Biotechnology VWR) and sodium chloride (Fisher Chemical).

Techniques: Adsorption, Membrane

Journal: Stem Cell Reports

Article Title: Scalable generation of T lymphocytes from human pluripotent stem cells in suspended organoids

doi: 10.1016/j.stemcr.2026.103076

Figure Lengend Snippet:

Article Snippet: Recombinant human fibroblast growth factor 2 (bFGF) , OriGene , Cat#TP750002.

Techniques: Recombinant